Blackwater Refuge offers several bike routes for the novice to experienced biker. Cyclists can choose to complete an approximate 4-mile or 7-mile-loop route along the paved Wildlife Drive. Blackwater also has a 20-mile and 25-mile route that runs along county roads through the varied habitats of the Refuge. Route maps can be obtained at the Visitor Center or a PDF version can be viewed here: Bike Map (120KB PDF file).

The roads in and around Blackwater Refuge are flat and near sea level. After a heavy rain, water may accumulate on some local roads outside the Refuge.

The Blackwater marsh supports a healthy insect population, so visitors are advised to bring insect repellent during warm weather.